Human IL-12(p35)(Interleukin 12 p35) ELISA Kit

Sample

Serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Human

Detection Method

sELISA

Intended Use

In vitro quantitative determination of IL-12 (p35) concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

1. ELISA Microplate(Dismountable) : 96T(8×12). Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C.
2. Lyophilized Standard: 2vial. Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C.
3. Biotin-labeled Antibody (Concentrated, 100×): 120 ul. 2-8°C (Avoid Direct Light)
4. HRP-Streptavidin Conjugate (SABC, 100×): 120 ul. 2-8°C (Avoid Direct Light)
5. TMB Substrate: 10 ml. 2-8°C (Avoid Direct Light)
6. Sample Dilution Buffer: 20 ml
7. Antibody Dilution Buffer: 10 ml
8. SABC Dilution Buffer: 10 ml
9. Stop Solution: 10 ml
10. Wash Buffer (25×): 30 ml
11. Plate Sealer: 5
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution.

Storage

Store at 2-8°C (for sealed box), please do not freeze! See kit label for expiry date.
Stability
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

31.25-2000pg/ml

Sensitivity

18.75pg/ml

General Description

IL-12 (p35) is a dimeric protein that forms the IL-12 (p70) molecule by associating with the p40 subunit. It is mainly produced by activated immune cells such as dendritic cells (DCs), macrophages, and B cells, and mediates the differentiation, proliferation, and activation of natural killer (NK) cells and T lymphocytes as a cytokine. IL-12 (p35) plays an important role in cellular immune responses and cytotoxic reactions, especially in the T helper 1 (Th1) response in cell-mediated immunity. IL-12 (p35) is also associated with the development of various diseases, such as autoimmune diseases, tumors, and infectious diseases.

Standard Curve

This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C - 25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.)
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.

General Notes

IL-12 (p35) is a dimeric protein that forms the IL-12 (p70) molecule by associating with the p40 subunit. It is mainly produced by activated immune cells such as dendritic cells (DCs), macrophages, and B cells, and mediates the differentiation, proliferation, and activation of natural killer (NK) cells and T lymphocytes as a cytokine. IL-12 (p35) plays an important role in cellular immune responses and cytotoxic reactions, especially in the T helper 1 (Th1) response in cell-mediated immunity. IL-12 (p35) is also associated with the development of various diseases, such as autoimmune diseases, tumors, and infectious diseases. In vitro quantitative determination of IL-12 (p35) concentrations in serum, plasma, cell culture supernatant and other biological samples.