Anti-SYP monoclonal antibody (DMAB5564RH)

Rabbit Anti-SYP monoclonal antibody for WB, ICC/IF, IHC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TK37-96
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:50-1:200 IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of Synaptophysin on different cell lysates using anti-Synaptophysin at 1/500 dilution. Positive control: Line 1: Human brain; Line 2: PC-12
Fig2: ICC staining Synaptophysin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Synaptophysin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.

Target


Alternative Names
Major synaptic vesicle protein p38; MRX96; MRXSYP; Syn p38; Synaptophysin; Syp

Citations


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References


GOTO, S; NAGAHIRO, S; et al. IMMUNOCYTOCHEMICAL DETECTION OF CALCINEURIN AND MICROTUBULE-ASSOCIATED PROTEIN-2 IN CENTRAL NEUROCYTOMA. JOURNAL OF NEURO-ONCOLOGY 16:19-24(1993).
Lee, JM; Fournel, M; et al. Association of CD45 with Lck and components of the Ras signalling pathway in pervanadate-treated mouse T-cell lines. ONCOGENE 12:253-263(1996).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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