Anti-SYP monoclonal antibody (DMAB5564RH)
Rabbit Anti-SYP monoclonal antibody for WB, ICC/IF, IHC
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Human, Mouse, Rat
Recommended Dilution: WB: 1:1,000-1:5,000; ICC: 1:50-1:200 IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Fig1: Western blot analysis of Synaptophysin on different cell lysates using anti-Synaptophysin at 1/500 dilution.
Line 1: Human brain;
Line 2: PC-12
Fig2: ICC staining Synaptophysin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Synaptophysin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
Major synaptic vesicle protein p38; MRX96; MRXSYP; Syn p38; Synaptophysin; Syp
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Shata, A; Saisu, H; et al. Phosphorylated synaphin/complexin found in the brain exhibits enhanced SNARE complex binding. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 354:808-813(2007).
Noguchi, T; Matozaki, T; et al. Characterization of a 115-kDa protein that binds to SH-PTP2, a protein-tyrosine phosphatase with Src homology 2 domains, in Chinese hamster ovary cells. JOURNAL OF BIOLOGICAL CHEMISTRY 271:27652-27658(1996).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.