Anti-HIF1A monoclonal antibody, clone 2B4

Name: Anti-HIF1A monoclonal antibody, clone 2B4
SKU: DCABH-11877

Mouse anti-Human HIF1A monoclonal antibody for WB, IHC-P, IF, ELISA

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COA

SDS

Datasheet

Summary

Specifications

Antibody Isotype

IgG1

Clone

2B4

Species Reactivity

Human; Monkey; Mouse

Immunogen

Recombinant protein corresponding to human HIF1A.

Conjugate

Unconjugated

Applications

Application Notes

ELISA (1:10000); WB (1:500-1:2000); Immunohistochemistry (1:200-1:1000); Immunofluorescence (1:200-1:1000) & The optimal working dilution should be determined by the end user.

Target

Alternative Names

HIF1A; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; bHLHe78; HIF 1alpha; HIF1; MOP1; PASD8; HIF-1-alpha; member of PAS protein 1; ARNT interacting protein; ARNT-interacting prot

Entrez Gene ID

3091

UniProt ID

Q16665

Product Background

Antigen Description

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.

Pathway

Adipogenesis, organism-specific biosystem; Angiogenesis, organism-specific biosystem; Circadian Clock, organism-specific biosystem; HIF-1-alpha transcription factor network, organism-specific biosystem; Hypoxic and oxygen homeostasis regulation of HIF-1-alpha, organism-specific biosystem; NOTCH1 Intracellular Domain Regulates Transcription, organism-specific biosystem; Notch-mediated HES/HEY network, organism-specific biosystem;

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

PAFAH1B2 is a HIF1a target gene and promotes metastasis in pancreatic cancer

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Authors: Ma, Can; Guo, Yan; Zhang, Yan; Duo, Aixia; Jia, Yitao; Liu, Ci; Li, Binghui

Abstract

Platelet-activating factor acetylhydrolase IB subunit beta (PAFAH1B2) plays important roles in inflammation and anaphylaxis. However, its primary function in pancreatic cancer remains unclear. In the current study, we report that PAFAHIB2 is overexpressed in pancreatic ductal adenocarcinoma (PDAC) and correlated inversely with patient survival. PAFAH1B2 overexpression induced epithelial mesenchymal transition (EMT), migration and invasion in vitro and metastasis in vivo. Conversely, silencing PAFAH1B2 inhibited these aggressive phenotypes. Moreover, PAFAH1B2 overexpression in PDAC cells was directly mediated by HIF1a. PAFAH1B2 expression in PDAC clinical specimens correlated positively with HIF1a expression. Overall, our results defined PAFAHIB2 as a target gene of HIF1a and a critical driver of PDAC metastatic behaviors. (C) 2018 The Authors. Published by Elsevier Inc.

HIF1A C1772T genetic variation is associated with the elevated risk of breast cancer among Asians: An updated meta-analysis

META GENE

Authors: Kancharla, Jyothsna; Dariya, Begum; Momin, Saimila; Prasad, Immadisetty Devi Vara; Bhaskar, L. V. K. S.; Bramhachari, Pallaval Veera; Alam, Afroz

Abstract

Breast cancer (BC) is considered to be one of the most malignant diseases. The microenvironment of BC is characterized as a hypoxic-like environment. Hypoxia inducible factor (HIF) is activated during hypoxic conditions, which can be generated by the BC microenvironment. Previous studies showed the relation between HIF genetic variations and BC, but were not well characterized. The objective of this meta-analysis is to analyze the relation of the HIF1A C1772T with BC by reviewing and analyzing established studies. All the data was gathered from nine studies that focused on HIF1A C1772T and BC. The literature was retrieved from electronic databases such as Web of Science, PubMed and Embase. All of the statistical data was subjected to either a fixed or random effects model to calculate OR and 95% confidence interval of pooled studies. The data was then analyzed by using the MetaGenyo web tool. This study did not find evidence for publication bias. The outcomes of the present study showed that HIF1A gene C1772T variant is not correlated with the risk of BC. However, sub-group studies by origin implied that the probability of BC was both strongly correlated with the HIF1A C1772T variant and significantly elevated among Asians. In summary, this study suggests that the probability of BC associated with the HIF1A C1772T variant was significantly higher in Asians compared to Caucasians.