Anti-GH1 monoclonal antibody, clone 8F8

Objectives: The aim of this work is to evaluate levels of placental growth hormone (PGH), pituitary growth hormone (OH 1), insulin-like growth factor (IGF-I) and ghrelin in pregnant women's blood serum before, during and after delivery. Furthermore, the aim is to search for links and interdependence of GH1, PGH and IGF-I concentrations. Material and methods: Seventy nine blood samples were taken one to two hours before, during and half an hour after expulsion of placenta. All proteins studied were determined by ELISA method, using ELISA Kit. Results: The highest PGH concentration and IGF-I concentration in pregnant women's blood serum was observed before delivery, while GH1 concentration was lowest. During and after delivery PGH and IGF-I concentration decreased proportionately and pituitary growth hormone concentration increased accordingly About half an hour after delivery of the placenta, GH1 concentration was highest. Conclusions: In pregnant women's blood there is a metabolic interdependence between PGH and IGF-I. Their concentration increases proportionately during pregnancy and decreases after delivery. It appears that labor and delivery releases GH1 blockade, which level rises three-fold during delivery After parturition its role and concentration returns to levels before pregnancy.

A novel Gram-reaction-negative bacterium, designated strain GH1-34(T), was isolated from a sample of tidal mudflat collected at the seashore of Gangwha Island, Republic of Korea. Cells of the bacterium were strictly aerobic, catalase- and oxidase-positive, motile by means of a polar flagellum and rod shaped. It was found to grow at 0-5 % (w/v) NaCl, 20-45 degrees C and pH 6-10. The major isoprenoid quinone was 0-10. The polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid and an unidentified glycolipid. The predominant fatty acids were C-18:1 omega 7c and C(19:0)cyclo omega 8c. The G+C content of the DNA was 70.9 mol%. Comparative 16S rRNA gene sequence analysis revealed that strain GH1-34(T) formed a tight cluster with the type strain of Lutibaculum baratangense with 98.3 % sequence similarity; levels of the 16S rRNA gene sequence similarity between the novel strain and other representatives of the order 'Rhizobiales' were <95.0 %. DNA-DNA relatedness between the organism and L. baratangense KCTC 22669(T) was 33 %, Based on the results of phenotypic analysis and DNA-DNA hybridization experiments, strain GH1-34(T) (=KCTC 52847(T)=NBRC 113277(T)) represents a novel species of the genus Lutibaculum, for which the name Lutibaculum pontilimi sp. nov. is proposed.