The NC1-H69 human small cell lung carcinoma line. The antibody binding epitope has been mapped to the first EGF-like repeat domain (EGF1) amino acids 27-59 of the EpCAM protein (Balzar, 1999; Balzar 2001).
Conjugate
Alexa Fluor 488
Applications
Application Notes
Flow Cytometry (5 μl/test/106 cells (0.2 ug)) & The optimal working dilution should be determined by the end user.
Target
Alternative Names
EPCAM; epithelial cell adhesion molecule; antigen identified by monoclonal AUA1 , M4S1, MIC18, TACSTD1, tumor associated calcium signal transducer 1; 17 1A; 323/A3; CD326; CO 17A; EGP 2; EGP34; EGP40; Ep CAM; ESA; GA733 2; HEA125; KS1/4; KSA; Ly74; MH99;
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Citations
Publication ()
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Q: What substance was used to create the Anti-EPCAM monoclonal antibody, clone WV2E0 [Alexa Fluor® 488]?
A: The NC1-H69 human small cell lung carcinoma line. The antibody binding epitope has been mapped to the first EGF-like repeat domain (EGF1) amino acids 27-59 of the EpCAM protein (Balzar, 1999; Balzar 2001).
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