Mouse Anti-Human EPCAM Hybridoma [273-32.3]

Immunogen

Human choriocarcinoma cell line BeWo

Fusion Species

Mouse X Mouse Hybridoma

Immunological Donor

Mouse spleen

Myeloma

NS-1

Clone

273-32.3

Cell Line Description

Animals were immunized with the human choriocarcinoma cell line BeWo. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with an antigen (Trop-1) present on trophoblast cells and on human choriocarcinomas.

Morphology

Lymphoblast

Growth Properties

Suspension

Subculturing

Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml

Storage

Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO

Synonyms

EPCAM; epithelial cell adhesion molecule; antigen identified by monoclonal AUA1, M4S1, MIC18, TACSTD1, tumor associated calcium signal transducer 1; 17 1A; 323/A3; CD326; CO 17A; EGP 2; EGP34; EGP40; Ep CAM; ESA; GA733 2; HEA125; KS1/4; KSA; Ly74; MH99; MK 1; MOC31; TACST 1; TROP1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); M4S1; MK-1; DIAR5; EGP-2; MIC18; EGP314; HNPCC8; TACSTD1; GA733-2;

Entrez Gene ID

UniProt ID