GeneralInformation
Immunogen
Human choriocarcinoma cell line BeWo
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Cell Line Description
Animals were immunized with the human choriocarcinoma cell line BeWo. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with an antigen (Trop-1) present on trophoblast cells and on human choriocarcinomas.
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Target
Synonyms
EPCAM; epithelial cell adhesion molecule; antigen identified by monoclonal AUA1, M4S1, MIC18, TACSTD1, tumor associated calcium signal transducer 1; 17 1A; 323/A3; CD326; CO 17A; EGP 2; EGP34; EGP40; Ep CAM; ESA; GA733 2; HEA125; KS1/4; KSA; Ly74; MH99; MK 1; MOC31; TACST 1; TROP1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); M4S1; MK-1; DIAR5; EGP-2; MIC18; EGP314; HNPCC8; TACSTD1; GA733-2;
Citations
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Gao, JJ; Yan, Q; et al. Epithelial-to-Mesenchymal Transition Induced by TGF-beta 1 Is Mediated by AP1-Dependent EpCAM Expression in MCF-7 Cells. JOURNAL OF CELLULAR PHYSIOLOGY 230:775-782(2015).
Gao, P; Pinkston, KL; et al. Deglycosylation of mAb by EndoS for Improved Molecular Imaging. MOLECULAR IMAGING AND BIOLOGY 17:195-203(2015).
COA
Certificate of Analysis
