Anti-eIF3E monoclonal antibody (DCABH-3148)

Rabbit anti-Human eIF3E monoclonal antibody for WB, IHC-P, ICC/IF, IP Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
FQS7989(3)
Species Reactivity
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues within Human eIF3e.
Conjugate
Unconjugated

Applications


Application Notes
WB: 1/1000 - 1/10000; IHC-P: 1/50 - 1/100; ICC/IF: 1/250 - 1/500; IP: 1/10 - 1/100;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
EIF3E; eukaryotic translation initiation factor 3, subunit E; EIF3S6, eukaryotic translation initiation factor 3, subunit 6 48kDa , INT6; eukaryotic translation initiation factor 3 subunit E; eIF3 p48; eIF3e
Entrez Gene ID
UniProt ID

Product Background


Pathway
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem; Cap-dependent Translation Initiation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; Formation of a pool of free 40S subunits, organism-specific biosystem; Formation of the ternary complex, and subsequently, the 43S complex, organism-specific biosystem; GTP hydrolysis and joining of the 60S ribosomal subunit, organism-specific biosystem; Gene Expression, organism-specific biosystem;

Citations


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References


Bandyopadhyay, A; Lakshmanan, V; et al. Moe1 and spInt6, the fission yeast homologues of mammalian translation initiation factor 3 Subunits p66 (eIF3d) and p48 (eIF3e), respectively, are required for stable association of eIF3 Subunits. JOURNAL OF BIOLOGICAL CHEMISTRY 277:2360-2367(2002).
Neusiedler, J; Mocquet, V; et al. INT6 interacts with MIF4GD/SLIP1 and is necessary for efficient histone mRNA translation. RNA-A PUBLICATION OF THE RNA SOCIETY 18:1163-1177(2012).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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