Rabbit Anti-Human EIF3E Polyclonal Antibody (DPABH-25989)
Rabbit Anti-Human EIF3E Polyclonal Antibody for IHC, WB, ICC-IF
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Human, Mouse, Rat
Recombinant Protein, antigen sequence: ARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY
ICC-IF: 1-4 µg/ml
IHC: 1:20 - 1:50
WB: 4 µg/ml
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine cells.
Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-EIF3E antibody. Remaining relative intensity is presented.
EIF3E; eukaryotic translation initiation factor 3, subunit E; INT6; EIF3S6; EIF3-P48; eIF3-p46
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Morris, C; Wittmann, J; et al. Human INT6/eIF3e is required for nonsense-mediated mRNA decay. EMBO REPORTS 8:596-602(2007).
Cai, Q; Todorovic, A; et al. Distinct Regions of Human eIF3 Are Sufficient for Binding to the HCV IRES and the 40S Ribosomal Subunit. JOURNAL OF MOLECULAR BIOLOGY 403:185-196(2010).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.