Anti-E. coli LexA DNA Binding Region Polyclonal antibody (DPABH-11143)
Rabbit Anti-E. coli LexA DNA Binding Region Polyclonal for WB; IP; ChIP; ICC/IF; IHC
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Recombinant full length protein corresponding to Escherichia coli LexA DNA Binding Region.Database link: POA7C2
WB: 1/1000 - 1/3000.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
LexA DNA Binding Region
The LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-termi.
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Movahedzadeh, F; Colston, MJ; et al. Determination of DNA sequences required for regulated Mycobacterium tuberculosis RecA expression in response to DNA-damaging agents suggests that two modes of regulation exist. JOURNAL OF BACTERIOLOGY 179:3509-3518(1997).
Sousa, FJR; Lima, LMTR; et al. Tetramerization of the LexA repressor in solution: Implications for gene regulation of the E-coli SOS system at acidic pH. JOURNAL OF MOLECULAR BIOLOGY 359:1059-1074(2006).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.