Anti-E. coli LexA DNA Binding Region Polyclonal antibody (DPABH-11143)

Rabbit Anti-E. coli LexA DNA Binding Region Polyclonal for WB; IP; ChIP; ICC/IF; IHC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
E. coli
Immunogen
Recombinant full length protein corresponding to Escherichia coli LexA DNA Binding Region.Database link: POA7C2
Conjugate
Unconjugated

Applications


Application Notes
WB: 1/1000 - 1/3000.
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
LexA DNA Binding Region

Product Background


Antigen Description
The LexA protein of Escherichia coli is a transcriptional repressor regulating approximately 20 genes, many of which are involved in DNA repair (SOS response). It binds with variable affinity to single, double, or even triple-operators via its amino-termi.

Citations


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References


Movahedzadeh, F; Colston, MJ; et al. Determination of DNA sequences required for regulated Mycobacterium tuberculosis RecA expression in response to DNA-damaging agents suggests that two modes of regulation exist. JOURNAL OF BACTERIOLOGY 179:3509-3518(1997).
Sousa, FJR; Lima, LMTR; et al. Tetramerization of the LexA repressor in solution: Implications for gene regulation of the E-coli SOS system at acidic pH. JOURNAL OF MOLECULAR BIOLOGY 359:1059-1074(2006).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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Order Info: Anti-E. coli LexA DNA Binding Region Polyclonal antibody

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