Anti-DPP4 monoclonal antibody (DCABH-2135)

Rabbit Anti-DPP4 monoclonal antibody for WB, ICC/IF, IHC, FC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
KN22-53
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:500-1:2,000; ICC: 1:50-1:200 IHC: 1:50-1:100; FC: 1:50-1:100
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of CD26 on mouse heart cells lysates using anti-CD26 antibody at 1/500 dilution.
Fig2: ICC staining CD26 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CD26 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD26 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-CD26 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of Hela cells with CD26 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody

Target


Alternative Names
CD26 antigen; ADA-binding protein; ADABP; ADCP 2; ADCP-2; ADCP2

Citations


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References


Harstad, EB; Rosenblum, JS; et al. DPP8 and DPP9 expression in cynomolgus monkey and Sprague Dawley rat tissues. REGULATORY PEPTIDES 186:26-35(2013).
Zhao, GY; Du, LY; et al. A safe and convenient pseudovirus-based inhibition assay to detect neutralizing antibodies and screen for viral entry inhibitors against the novel human coronavirus MERS-CoV. VIROLOGY JOURNAL 10:-(2013).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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