General Notes
This product (Catalog No. DPABH-03596) is a rabbit-derived polyclonal antibody raised against human DLAT, targeting the amino acid region 374–631 of the protein. This antibody exhibits cross-reactivity with Human DLAT from mouse and rat. It has been affinity purified by Protein A. Dihydrolipoyl transacetylase (EC 2.3.1.12), also known as dihydrolipoamide S-acetyltransferase, is the E2 subunit of the pyruvate dehydrogenase complex (PDC), a large multi-enzyme assembly in the mitochondrial matrix that catalyzes the oxidative decarboxylation of pyruvate to acetyl-coenzyme A (acetyl-CoA), thereby linking glycolysis to the tricarboxylic acid (TCA) cycle. In humans, the enzyme is encoded by the DLAT gene on chromosome 11 and consists of 647 amino acids with a molecular weight of approximately 69 kDa. It performs the second step of the PDC reaction by transferring an acetyl group from S-acetyldihydrolipoamide—covalently bound to lysine residues on its own lipoyl domains—to coenzyme A (CoA), yielding acetyl-CoA and dihydrolipoamide, which is subsequently reoxidized by the E3 subunit. This transacetylation is essential for efficient substrate channeling within the complex, preventing the release of reactive intermediates into the cellular environment and enhancing overall catalytic efficiency.
DPABH-03596 is suitable for use in ELISA (1:4,000–1:8,000), IHC (1:50–1:100), Western blot (WB) (1:1,000–1:4,000) applications.
DPABH-03596 is supplied as a liquid formulation in 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. For long-term storage, keep at −20 °C. For short-term use, store at 2–8 °C. Avoid repeated freeze–thaw cycles to maintain antibody integrity and performance.
Alternative Names
DLTA; M2 antigen complex 70 kDa subunit; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; Pyruvate dehydrogenase complex component E2; PDC-E2; DLAT; PBC; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; mitochondrial; PDCE2
COA
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