Anti-DLAT monoclonal antibody (DCABH-302)

Mouse anti-Pig DLAT monoclonal antibody for WB, ICC/IF, FC, IHC-P, ELISA Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
26E4H0D22
Species Reactivity
Cow, Human
Immunogen
Porcine Pyruvate Dehydrogenase E2 protein.
Conjugate
Unconjugated

Applications


Application Notes
WB: 0.5 μg/ml; ICC/IF: 0.2 - 0.5 μg/ml; Flow Cyt: 1 μg/ml; IHC-P: 1/100; In-Cell ELISA: 1 μg/ml;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
DLAT; dihydrolipoamide S-acetyltransferase; dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; dihydrolipoamide acetyltransferase; dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydr
Entrez Gene ID
UniProt ID

Product Background


Gene summary
DLAT (Dihydrolipoamide S-Acetyltransferase) is a Protein Coding gene. Diseases associated with DLAT include pyruvate dehydrogenase e2 deficiency and primary biliary cirrhosis. Among its related pathways are Signaling by GPCR and Metabolism. GO annotations related to this gene include transferase activity, transferring acyl groups and dihydrolipoyllysine-residue acetyltransferase activity. An important paralog of this gene is DBT. This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.
Pathway
Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem; Glycolysis / Gluconeogenesis, organism-specific biosystem; Glycolysis / Gluconeogenesis, conserved biosystem; Metabolic pathways, organism-specific biosystem; Pyruvate metabolism, organism-specific biosystem; Pyruvate metabolism, conserved biosystem.

Citations


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References


Tian, J; Bryk, R; et al. Mycobacterium tuberculosis appears to lack alpha-ketoglutarate dehydrogenase and encodes pyruvate dehydrogenase in widely separated genes. MOLECULAR MICROBIOLOGY 57:859-868(2005).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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