Rabbit Anti-DICER1 Polyclonal Antibody (DPABH-02262)
Rabbit Anti-Human DICER1 Polyclonal Antibody for WB, IHC
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Recombinant funsion protein containing a sequence corresponding to amino acids 1200-1460 of human DICER1 (NP_085124.2).
WB: 1:500 - 1:1000; IHC: 1:50 - 1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Immunohistochemistry of paraffin-embedded human lung cancer using DICER1 antibody at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using DICER1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
DICER1; dicer 1, ribonuclease type III; DCR1; MNG1; Dicer; HERNA
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Belver, L; de Yebenes, VG; et al. MicroRNAs Prevent the Generation of Autoreactive Antibodies. IMMUNITY 33:713-722(2010).
Helland, A; Anglesio, MS; et al. Deregulation of MYCN, LIN28B and LET7 in a Molecular Subtype of Aggressive High-Grade Serous Ovarian Cancers. PLOS ONE 6:-(2011).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.