Rabbit Anti-DICER1 Polyclonal Antibody (DPABH-02262)

Rabbit Anti-Human DICER1 Polyclonal Antibody for WB, IHC Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Species Reactivity
Human, Mouse
Immunogen
Recombinant funsion protein containing a sequence corresponding to amino acids 1200-1460 of human DICER1 (NP_085124.2).
Conjugate
unconjugated

Applications


Application Notes
WB:    1:500 - 1:1000
IHC:    1:50 - 1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Immunohistochemistry of paraffin-embedded human lung cancer using DICER1 antibody at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using DICER1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Target


Alternative Names
DICER1; dicer 1, ribonuclease type III; DCR1; MNG1; Dicer; HERNA
Entrez Gene ID
UniProt ID

Citations


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References


Jafarnejad, SM; Sjoestroem, C; et al. Expression of the RNase III enzyme DROSHA is reduced during progression of human cutaneous melanoma. MODERN PATHOLOGY 26:902-910(2013).
Helland, A; Anglesio, MS; et al. Deregulation of MYCN, LIN28B and LET7 in a Molecular Subtype of Aggressive High-Grade Serous Ovarian Cancers. PLOS ONE 6:-(2011).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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