Anti-DAG1 monoclonal antibody, clone 54EBH2/9E6

Antibody Isotype

IgG2a

Clone

54EBH2/9E6

Species Reactivity

Mouse; Rat; Rabbit; Chicken; Hamster; Dog; Human; Xenopus laevis; Zebrafish

Immunogen

Synthetic peptide: PKNMTPYRSPPPYVP, containing 15 of the last 16 amino acids at the extreme C terminus of Human beta Dystroglycan

Conjugate

Application Notes

IHC-Fr: 1/50 - 1/200; IHC-P: 5 μg/ml.

Alternative Names

DAG1; dystroglycan 1 (dystrophin-associated glycoprotein 1); dystroglycan; 156DAG; A3a; AGRNR; alpha dystroglycan; beta dystroglycan; DAG; dystrophin associated glycoprotein 1; MDDGC7; FLJ51254;

Entrez Gene ID

UniProt ID

Antigen Description

Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation.

Pathway

Arrhythmogenic right ventricular cardiomyopathy (ARVC), organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), conserved biosystem; Dilated cardiomyopathy, organism-specific biosystem; Dilated cardiomyopathy, conserved biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Hypertrophic cardiomyopathy (HCM), organism-specific biosystem;