Anti-CCND1 monoclonal antibody (DMAB5801RH)

Rabbit Anti-CCND1 monoclonal antibody for WB, ICC/IF, IHC, IP Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
TB49-19
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein
Conjugate
Unconjugated

Applications


Application Notes
Recommended Dilution: WB: 1:1000-1:5,000; ICC: 1:50-1:200; IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Fig1: Western blot analysis of Cyclin D1 on different lysates using anti-Cyclin D1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela; Lane 2: PC-12 Lane 3: SH-SY-5Y
Fig2: ICC staining Cyclin D1 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.

Target


Alternative Names
AI327039; B cell CLL/lymphoma 1; B cell leukemia 1; B cell lymphoma 1 protein; B-cell lymphoma 1 protein; BCL 1
Entrez Gene ID

Citations


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References


Vallbohmer, D; Zhang, W; et al. Molecular determinants of cetuximab efficacy. JOURNAL OF CLINICAL ONCOLOGY 23:3536-3544(2005).
Nai, G; Marques, M; et al. Role of ROC1 protein in the control of cyclin D1 protein expression in skin melanomas. PATHOLOGY RESEARCH AND PRACTICE 207:174-181(2011).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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