Anti-CCND1 monoclonal antibody (DMAB5801RH)
Rabbit Anti-CCND1 monoclonal antibody for WB, ICC/IF, IHC, IP
Datasheet
Specifications
Host Species
Rabbit
Antibody Isotype
IgG
Clone
TB49-19
Species Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein
Conjugate
Unconjugated
Applications
Application Notes
Recommended Dilution: WB: 1:1000-1:5,000; ICC: 1:50-1:200; IHC: 1:50-1:200
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Data Examples
Fig1: Western blot analysis of Cyclin D1 on different lysates using anti-Cyclin D1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela;
Lane 2: PC-12
Lane 3: SH-SY-5Y
Fig2: ICC staining Cyclin D1 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
Target
Alternative Names
AI327039; B cell CLL/lymphoma 1; B cell leukemia 1; B cell lymphoma 1 protein; B-cell lymphoma 1 protein; BCL 1
Entrez Gene ID
Citations
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References
Bobos, M; Kotoula, V; et al. Aberrant CCND1 copies and cyclin D1 mRNA expression do not result in the production of functional cyclin D1 protein in anaplastic large cell lymphoma. HISTOLOGY AND HISTOPATHOLOGY 24:1035-1048(2009).
Dedes, KJ; Lopez-Garcia, MA; et al. Cortactin gene amplification and expression in breast cancer: a chromogenic in situ hybridisation and immunohistochemical study. BREAST CANCER RESEARCH AND TREATMENT 124:653-666(2010).
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Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.
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