Magic™ Anti-hCG alpha monoclonal antibody, clone N83728

The detection of chlorogenic acid in clinic samples for metabolic kinetics study is quite a challenging task due to the low concentration and the lacking of sensitive quantitation methods. In this work, a porous pencil lead electrode (PLE) has been used to detect chlorogenic acid by square wave voltammetry (SWV) technique. The redox potential of chlorogenic acid is at ca. + 0.42V (pH = 3.0) versus saturated calomel electrode (SCE), and it's a reversible thin layer electrochemical process with two-electrons and two-protons involved in this reaction. The sensitivity was significantly improved due to the enrichment of CGA at the porous structure of the electrode. Under the optimized conditions, the concentration of chlorogenic acid was linear in the range of 7.7 x 10(-8) similar to 7.7 x 10(-6) M with detection limits down to 4.5 x 10(-9) M (R-2 = 0.9978, S/N = 3). The electrode was successfully used for the determination of chlorogenic acid in human urine with near 100 % recovery. Due to the advantages of simplicity of fabrication, high sensitivity, good reproducibility and easy renewability, we believe that the porous PLE is quite a promising candidate for determination of chlorogenic acid in clinic samples.

To determine the Dysgonia stuposa mitochondrial genome (mitogenome) structure and to clarify its phylogenetic position, the entire mitogenome of D. stuposa was sequenced and annotated. The D. stuposa mitogenome is 15,721 bp in size and contains 37 genes (protein-coding genes, transfer RNA genes, ribosomal RNA genes) usually found in lepidopteran mitogenomes. The newly sequenced mitogenome contained some common features reported in other Erebidae species, e.g., an A+T biased nucleotide composition and a non-canonical start codon for cox1 (CGA). Like other insect mitogenomes, the D. stuposa mitogenome had a conserved sequence 'ATACTAA' in an intergenic spacer between trnS2 and nad1, and a motif `ATAGA' followed by a 20 bp poly-T stretch in the A+T rich region. Phylogenetic analyses supported D. stuposa as part of the Erebidae family and reconfirmed the monophyly of the subfamilies Arctiinae, Catocalinae and Lymantriinae within Erebidae.