Anti-ATM (aa 1979-1983) polyclonal antibody

Antibody Isotype

IgG

Species Reactivity

Mouse; Human

Immunogen

Synthetic peptide conjugated to KLH, derived from around amino acids 1979-1983 (EGSQS) of Human ATM (NP_000042.3).

Conjugate

Application Notes

WB: 1/500 - 1/1000; ICC/IF: 1/100 - 1/200.

Alternative Names

ATM; ataxia telangiectasia mutated; ATA, ataxia telangiectasia mutated (includes complementation groups A, C and D); ATC, ATD, ATDC; serine-protein kinase ATM; TEL1; telomere maintenance 1; homolog (S. cerevisiae); TELO1; AT mutated; A-T mutated; TEL1, te

Entrez Gene ID

UniProt ID

Antigen Description

Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates Ser-139 of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends.

Pathway

ATM mediated phosphorylation of repair proteins; ATM mediated response to DNA double-strand break; Apoptosis; Autodegradation of the E3 ubiquitin ligase COP1; BARD1 signaling events; BRCA1-associated genome surveillance complex (BASC);