Anti-AKT1 monoclonal antibody (DCABH-59)

Mouse Anti-Human AKT1 monoclonal antibody for IP, WB, ELISA, IHC-P Datasheet

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Specifications


Host Species
Mouse
Antibody Isotype
IgG1
Clone
29G4.I22
Species Reactivity
Mouse, Human
Immunogen
Synthetic peptide corresponding to residues surrounding the internal sequence amino acid T308 of Human AKT1 protein.
Conjugate
Unconjugated

Applications


Application Notes
WB: 1/500 - 1/3000; ELISA: 1/20000; IHC-P: 20 μg/ml;
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
AKT1; v-akt murine thymoma viral oncogene homolog 1; RAC-alpha serine/threonine-protein kinase; AKT; PKB; PRKBA
Entrez Gene ID
UniProt ID

Product Background


Pathway
AKT phosphorylates targets in the cytosol, organism-specific biosystem; AKT phosphorylates targets in the nucleus, organism-specific biosystem; AKT-mediated inactivation of FOXO1A, organism-specific biosystem; Activation of BAD and translocation to mitochondria, organism-specific biosystem; Activation of BH3-only proteins, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem;

Citations


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References


Sundstrom, JM; Sundstrom, CJ; et al. Phosphorylation Site Mapping of Endogenous Proteins: A Combined MS and Bioinformatics Approach. JOURNAL OF PROTEOME RESEARCH 8:798-807(2009).
Byun, HJ; Hong, IK; et al. A splice variant of CD99 increases motility and MMP-9 expression of human breast cancer cells through the AKT-, ERK-, and JNK-dependent AP-1 activation signaling pathways. JOURNAL OF BIOLOGICAL CHEMISTRY 281:34833-34847(2006).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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