Vitamin B6 ELISA Kit

Sample

Cereals (maize meal, soybean meal, millet flour, rice flour), milk, milk powder

Intended Use

The Vitamin B6 ELISA Kit is used for quantitative analysis of Vitamin B6 in cereals (maize meal, soybean meal, millet flour, and rice flour), milk, milk powder and other food preparations.

Storage

1. All reagents should be stored at 2ºC to 8ºC for stability.
2. All the reagents and wash solutions should be used within 12 months from manufacturing date.
3. Before using, bring all components to room temperature (18-25ºC). Upon assay completion ensure all components of the kit are returned to appropriate storage conditions.
4. The Substrate is light-sensitive and should be protected from direct sunlight or UV sources.

Precision

Intra-Assay: CV<18%
Inter-Assay: CV<20%

Detection Range

5ng/ml - 80ng/ml

Detection Limit

It is defined as the lowest detectable concentration corresponding to a signal of Mean of ‘0’ standard plus 2* SD. 10 replicates of ‘0’ standards were evaluated and the LOD was found to be 2 ng/ml.

General Description

Vitamin B6 refers to a group of chemically similar compounds which can be interconverted in biological systems. Vitamin B6 is part of the vitamin B group of essential nutrients. Its active form, pyridoxal 5′- phosphate, serves as a coenzyme in some 100 enzyme reactions in amino acid, glucose, and lipid metabolism.