VB1 ELISA Kit

Sample

Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids

Species Reactivity

Universal

Detection Method

cELISA

Intended Use

For the quantitative measurement of VB1 in biological fluids. This product is intended for research use only.

Storage

Open kit immediately upon receipt. Store components at -20°C (NOTE: exceptions below) for 6 months or until expiration date. Avoid any freeze/thaw cycles.

Precision

Mean Intra-assay CV%: < 6.8% (n = 20)
Mean Inter-assay CV%: < 8.4% (n = 20)

Detection Range

1.56-100ng/mL

Sensitivity

0.36 ng/mL

General Description

Thiamine is a heat-labile and water-soluble essential vitamin, belonging to the vitamin B family, with antioxidant, erythropoietic, mood modulating, and glucose-regulating activities. Thiamine reacts with adenosine triphosphate (ATP) to form an active coenzyme, thiamine pyrophosphate. Thiamine pyrophosphate is necessary for the actions of pyruvate dehydrogenase and alpha-ketoglutarate in carbohydrate metabolism and for the actions of transketolase, an enzyme that plays an important role in the pentose phosphate pathway. Thiamine plays a key role in intracellular glucose metabolism and may inhibit the action of glucose and insulin on arterial smooth muscle cell proliferation. Thiamine may also protect against lead toxicity by inhibiting lead-induced lipid peroxidation.