Specifications
Immunogen
V5 tag fusion protein
Applications
Application Notes
WB 1:5,000-1:20,000; ELISA 1:5,000-1:20,000; ICC/IF 1:200-1:1000; Flow Cyt 1μg for 10^6 cells
Each laboratory should determine an optimum working titer for use in its particular application.
Other applications have not been tested but use in such assays should not necessarily be excluded.
General Notes
Anti-V5 tag, HiResNb™ VHH-Rabbit Fc Antibody(AF647) is designed for detecting V5 tag fusion proteins specifically. Anti-V5 tag, HiResNb™ VHH-Rabbit Fc Antibody(AF647) is based on monoclonal, recombinant, rabbit IgG Fc fused single domain Antibody to V5 tag coupled to AF647. Based on immunoelectrophoresis and/or ELISA, Anti-V5 tag, HiResNb™ VHH-Rabbit Fc Antibody(AF647) detects the V5 tag selectively, no reactivity with other proteins. Immunogen:V5 tag fusion protein
Target
Alternative Names
GKPIPNPLLGLDST epitope tag; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope tag; V5 Tag; Simian Virus 5 Tag
Citations
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Ooms, K; Van Gorp, H; et al. Development of a recombinant antibody to target peptides and proteins to sialoadhesin-expressing macrophages. BMC BIOTECHNOLOGY 13:-(2013).
Jenkins, RW; Idkowiak-Baldys, J; et al. A Novel Mechanism of Lysosomal Acid Sphingomyelinase Maturation REQUIREMENT FOR CARBOXYL-TERMINAL PROTEOLYTIC PROCESSING. JOURNAL OF BIOLOGICAL CHEMISTRY 286:3777-3788(2011).
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COA
Certificate of Analysis
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