Total Phenols Colorimetric Assay Kit

Sample

Plant Samples

Intended Use

This kit can be used to measure the total phenols content in plant tissue samples.

Contents of Kit

Reagent 1, Folin Phenol Reagent, 60 mL × 2 vials, 2-8°C, 12 months, shading light
Reagent 2, Alkali, Powder × 4 vials, 2-8°C, 12 months
Reagent 3, O-dihydroxybenzene, 10 mg × 8 vials, 2-8°C, 12 months, shading ligh
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

Storage

2-8°C

Precision

Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)

Inter-assay Precision
Three human serum samples were assayed 17 times in duplicate by three operators to determine precision between assays.

Sensitivity

The analytical sensitivity of the assay is 0.73 μg/mL. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.

Standard Curve

As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only: