Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Total Phenols content in urine, serum, plasma, tissue extracts, cell lysate, cell culture and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Reaction Buffer | 3 mL x 1 | 4°C |
| Dye Reagent | 1 mL x 1 | 4°C |
| Standard | Powder x 1 | 4°C |
| Plate Sealer | 3 | |
| Instruction Manual | 1 | |
Detection Range
0.04 mmol/L - 4 mmol/L
General Description
Phenols constitute probably the largest group of plant secondary metabolites, varying in size from a simple structure with an aromatic ring to complex ones such as lignins. Although many of the essential oils are terpenes, some are phenolic compounds. Many simple phenols are responsible for taste. They are called the phenylpropanoids because they originate from phenylalanine, and they have a six-carbon (C6) and three- carbon (C3) structure. Total Phenols Microplate Assay Kit is a sensitive assay for determining total phenols content in various samples. Phenols can react with phosphomolybdic acid, and the product can be measured at a colorimetric readout at 760 nm, is proportional to the phenols concentration in the sample.
Citations
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