Tocilizumab ELISA Kit

Sample

Serum, Plasma (EDTA, Heparin)

Detection Method

sELISA

Intended Use

Enzyme immunoassay for determination of quantitative antibodies to Tocilizumab in serum and plasma samples

Contents of Kit

1. Microtiter Plate: 1 × 12 × 8, Break apart strips. Microtiter plate with 12 rows each of 8 wells coated with reactant.
2. Standard A-E: 0.3 mL (each)
Standard A: 3000 ng/mL
Standard B: 1000 ng/mL
Standard C: 300 ng/mL
Standard D: 100 ng/mL
Standard E: 0 ng/mL
Ready to use. Used for the standard curve and control. Contains tocilizumab, human serum and stabilizer, < 0,1 % NaN₃.
3. Control low and high levels: 0.3 mL (each) Ready to use. Contains human serum and stabilizer, < 0,1 % NaN₃ Control concentrations are given in COA.
4. Assay Buffer: 2 × 50 mL, Ready to use. Blue coloured. Contains proteins, < 0,1 % NaN₃.
5. Conjugate: 1 × 12 mL, Horse radish peroxidase. Conjugated probe ready to use. Red coloured. Contains HRP conjugated probe, stabilizer and preservatives.
6. Substrate: 1 × 12 mL, TMB substrate solution. Ready to use. Contains 3,3′,5,5′- Tetramethylbenzidine (TMB).
7. Stop Buffer: 1 × 12 mL, Ready to use. 1N HCl.
8. Wash Buffer (20×): 1 × 50 mL, Prepared concentrated (20×) and should be diluted with the dilution rate given in the "Reagent Preparation" before the test. Contains buffer with tween 20.
9. Foil: 2 × 1, For covering microtiter plate during incubation.

Storage

The kit is shipped at ambient temperature (10-30°C) and should be stored at 2-8°C for long term storage. Keep away from heat or direct sunlight. The strips of microtiter plate are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2-8°C.

Precision

Intra-assay and inter-assay CVs < 30%

Sensitivity

The lowest detectable level (Lowest detection limit, LOD) that can be distinguished from the zero standard is 0,003 μg/ml
Functional sensitivity (Limit of quantification-LOQ): 0,01 μg/mL

General Description

Tocilizumab, also known as atlizumab, is an immunosuppressive drug, mainly for the treatment of rheumatoid arthritis (RA) and systemic juvenile idiopathic arthritis, a severe form of arthritis in children. It is a humanized monoclonal antibody against the interleukin-6 receptor (IL-6R). Interleukin 6 (IL-6) is a cytokine that plays an important role in immune response and is implicated in the pathogenesis of many diseases, such as autoimmune diseases, multiple myeloma and prostate cancer. It was developed by Hoffmann–La Roche and Chugai.

General Notes

Other ELISA Kits