Terramycin Standard

In this work, we designed and synthesized a nanocomposite comprising an amine-functionalized metal organic framework (UiO-66-NH2), a multiwalled carbon nanotube@reduced graphene oxide nanoribbon (MWCNT@rGONR) and a covalent organic framework (COF) synthesized using melamine and cyanuric acidmonomers via polycondensation (represented by MCA). The UiO-66-NH2/MCA/MWCNT@rGONR nanocomposite was used as a sensitive platform for an electrochemical aptasensor to detect kanamycin (kana). Owing to the rich chemical functionality, amino-rich structure and excellent electrochemical activity, the cDNA strands with terminal amino groups can not only anchor over the UiO-66-NH2/MCA/MWCNT@rGONR surface but also penetrate into the interior of porous UiO-66-NH2/MCA/MWCNT@rGONR networks. The characterization of the UiO-66-NH2/MCA/MWCNT@rGONR nanocomposite was performed by scanning electronic microscopy (SEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffraction (XRD). Furthermore, cyclic voltammetry (CV) and square wave voltammetry (SWV) were employed for the electrochemical performance study of this biosensor. The results indicated that the UiO-66-NH2/MCA/MWCNT@rGONR nanocomposite exhibited high bioaffinity toward the aptamer and the lowest limit of detection at 13 nM (S/N = 3) within a linearity of the kana concentration of 25-900 nM. In addition, it possessed great repeatability, stability and selectivity and obtained satisfactory recovery results in the real analysis of fish meat and milk, indicating the great potential for analytical measurements in food safety.

In this study, cetyl trimethylammonium bromide (CTAB) (cationic) and sodium dodecyl benzene sulfonate (SDBS) (anionic) were used to modify natural sepiolite (SEP) to obtain a type of organic sepiolite (C-S-SEP). It was further applied for adsorption of oxytetracycline (OTC), a common antibiotic in water. The changes of SEP crystal structure and physicochemical properties before and after modification were analyzed by the means of XRD, FTIR, TG, SEM/EDS, BET, XPS and zeta potential. The adsorption performance and mechanism of OTC on C-S-SEP were studied by static adsorption method. The results showed that the adsorption capacity of C-S-SEP increased significantly, and the removal rate of OTC increased from 50.26% to 99.42%. The partition coefficient of SEP and C-S-SEP was 0.356 and 2.172 mg g(-1) mu M-1, respectively. CTAB and SDBS were successfully loaded onto the surface of SEP without entering its inter-laminar domain, and the original crystal structure of SEP was well maintained. In the range of the studied ratio, anionic and cationic surfactants had the synergistic solubilization effect. The adsorption process conformed to the pseudo-second-order kinetic model and Langmuir isothermal adsorption model. The adsorption reaction was exothermic and a process of entropy reduction. The increase of temperature was not conducive to adsorption, and the adsorption reaction was basically unaffected by the pH value. The adsorption of C-S-SEP on OTC was the result of the combination of distribution and surface adsorption. The organic modified SEP was expected to become a low-cost environmentally friendly adsorption material that can effectively remove OTC from water. (C) 2019 Elsevier B.V. All rights reserved.