Anti-Human AS160 (phospho Thr642) monoclonal antibody (CABT-L1627)

Rabbit Anti-Human AS160 (phospho Thr642) monoclonal antibody for WB Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
20I29M8
Species Reactivity
Human
Immunogen
Peptide with the sequence RRRAH[pT]FSHPPS corresponding to amino acids 637-648 of AS160
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution:
WB: 2-3 µg/ml
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Western blot analysis of Phospho-AS160 pThr642 in whole cell extract (80ug per lane) from HeLa cells (serum-starved) treated with IGF1 (150 ng/mL, 15 min) using a Phospho-AS160 pThr642 recombinant rabbit monoclonal antibody at a dilution of 2.5 µg/mL. Detection was performed using an HRP-conjugated goat anti-rabbit secondary antibody at a dilution of 1:5000 followed by chemiluminescence (ECL). Results show a band at ~160kDa.

Target


Alternative Names
TBC1D4; TBC1 domain family, member 4; AS160; TBC1 domain family member 4; akt substrate of 160 kDa; Acrg embryonic lethality minimal region ortholog

Citations


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References


Treebak, JT; Taylor, EB; et al. Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY 298:C377-C385(2010).
Funai, K; Schweitzer, GG; et al. In vivo exercise followed by in vitro contraction additively elevates subsequent insulin-stimulated glucose transport by rat skeletal muscle. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM 298:E999-E1010(2010).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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