Sulfamethoxazole ELISA Test Kit

Sample

Eggs, fish, shrimp, meat (beef, chicken and pork), honey, milk, milk powder

Detection Method

cELISA

Intended Use

Sulfamethoxazole ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Sulfonamide in eggs, fish, shrimp, meat (beef, chicken and pork), honey, milk, milk powder.
The unique features of the kit are:
(1) Rapid extraction method for various samples with high recovery (80-120%).
(2) High reproducibility.
(3) A quick ELISA assay (less than 1 hours regardless of number of samples).

Contents of Kit

Sulfamethoxazole ELISA Test Kit has the capacity for 96 determinations of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package.
1. Sulfamethoxazole Plate: 1x96-well
2. Sulfamethoxazole Standards: 0.5ng/mL, 1.5 ng/mL, 4.5 ng/mL, 13.5ng/mL, 40.5 ng/mL, 1000 ng/mL, 1.0 mL for each.
3. Negative control: 1.0 mL
4. Sulfamethoxazole Antibody #1: 6 mL
5. HRP Conjugate Antibody #2: 6 mL
6. 10X Sample Extraction Buffer: 30 mL
7. 20X Wash Solution: 30 mL
9. Stop Buffer: 12 mL
10. TMB Substrate: 12 mL
* If you are not planning to use the kit for over 1 month, store Sulfamethoxazole Antibody #1, HRP Conjugate Antibody #2 at -20°C or in a freezer.

Storage

Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.

Detection Limit

Egg: 0.5ppb
Fish/shrimp/meat/liver/kidney: 0.5ppb
Honey:5ppb
Milk:0.5ppb
Milk Powder:2.5ppb

Standard Curve

The following figure is a typical Sulfamethoxazole standard curve. The sample detection and quantification limit are calculated as below.
Sample detection limit = (0.5 ng/g) x (dilution factor)
Sample quantification limit = (1.5ng/g) x (dilution factor)