SF9 HCP ELISA Kit

Name: SF9 HCP ELISA Kit
SKU: DEIASL170
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Insect cell-baculovirus expression system

Detection Method

sELISA

Intended Use

It' s applicable to biological products based on insect cell-baculovirus expression system, including but not limited to recombinant proteins, vaccines, gene therapy AAV vectors, etc.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Sf9 HCP Standard	Lyophilized powder × 3 vials	2-8°C
2	Coated Plate	8 wells × 12 strips	2-8°C
3	Standard Reconstitution Solution	1.5 mL × 2 tubes	2-8°C
4	Dilution Buffer	50 mL × 1 vial	2-8°C
5	Concentrated Buffer (10×)	25 mL × 2 vials	2-8°C
6	Sf9 HCP Enzyme-labeled Antibody (100×)	120 μL × 1 tube	2-8°C
7	TMB Substrate	12 mL × 1 vial	2-8°C
8	Stop Solution	6 mL × 1 vial	2-8°C
9	Sealing Film	5 pieces	2-8°C
10	Instructions for Use	1 copy

Storage

Sealed kits are valid for 12 months at 2-8°C.
Storage requirements for unsealed components are as follows:
Microtiter plate: The unsealed microtiter plate strip shall be sealed and stored in a zip lock bag together with desiccant, and it can be stable for 30 days at 2-8°C after validation.
Reconstitution of calibrator: The calibrator can be stored at 2-8°C on the day of dissolution. After use, transfer to ≤ -18°C for storage on the day of use. Repeated freezing and thawing should not exceed 3 times.

Standard Curve

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Q & A

Ask a Question

Q: How have the cells been lysed to release proteins for raising the antibodies? Would intranuclear proteins be released?

A: In antibody production, cell lysis aims to disrupt cell structure and release cellular contents (including proteins). Common methods include physical lysis, chemical lysis, and enzymatic lysis.
During cell lysis, nucleoproteins may be released. If the lysis method is sufficiently drastic (such as the lysis conditions used during kit development), nucleoproteins will be released along with other HCPs.
The polyclonal antibodies used in this SF9 HCP ELISA kit recognize a variety of HCPs, including nucleoproteins, making it suitable for comprehensive residual protein detection in biological products (such as vaccines and AAV vectors).
Note: In actual testing, to ensure effective detection of nucleoproteins, sample pretreatment must follow the lysis steps specified in the kit.

Customer Reviews