SF9 HCP ELISA Kit

Name: SF9 HCP ELISA Kit
SKU: DEIABY261
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Biological products

Detection Method

Sandwich-ELISA

Intended Use

SF9 HCP ELISA kit is intended for quantitative detection of Sf9 host cell protein residues in protein purification processes and final products. This kit is for research and production use only. Not for clinical or diagnostic procedures.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Pre-coated Microplate	96T × 1 plate (strips)	2-8°C (Sealed)
2	Standard Stock Solution (500 μg/mL)	50 μL × 1 vial	2-8°C
3	Detection Antibody (100×)	150 μL × 1 vial	2-8°C
4	TMB Substrate	10 mL × 1 bottle	2-8°C (Protect from light)
5	Stop Solution	10 mL × 1 bottle	2-8°C
6	Wash Buffer (20×)	10 mL × 5 bottles	2-8°C
7	Diluent (10×)	10 mL × 1 bottle	2-8°C
8	Plate Sealer	4 sheets

Storage

Store kit at 2 - 8°C. Do not use kit after expiration date.

Performance Characteristics

Advantages
• Broad Coverage: The antibodies possess strong HCP recognition capability, achieving >80% coverage with stable performance.
• High Titer: The antibodies used in this kit demonstrate a titer of up to 10⁶ when tested by indirect ELISA.
• High Sensitivity: Serum antibodies are purified using affinity chromatography to minimize non-specific antibodies.
• High Stability: The production process incorporates broad-spectrum protein stabilizers and specialized microplate treatment to enhance the thermal stability of standards and plates, ensuring result reproducibility.
• Robust Applicability: The kit has been tested under varying reaction temperatures (20-30°C) and incubation times (±10 minutes), showing good reproducibility.
• Optimized Diluent: The use of an optimized sample diluent reduces non-specific adsorption during sample testing, resulting in very low background color, which facilitates accurate concentration determination.

Precision

Intra-assay CV%: 4 - 6%
Inter-assay CV%: 2 - 7%

Sensitivity

LOD: 1.56 ng/mL
LOQ: 6.25 ng/mL

General Description

Recombinant expression using Sf9 insect cells is a common, efficient, and economical method for obtaining target proteins or viruses. As these products are often used in human and animal treatments, high purification is required. Residual Sf9 host cell proteins (HCP) can trigger adverse toxic or immune reactions. Removing these potential hazards is a primary goal of downstream biopharmaceutical processing.
Enzyme-Linked Immunosorbent Assay (ELISA) is the "gold standard" for HCP detection designated by global regulatory agencies due to its high sensitivity.

Standard Curve