Product Overview
Recombinant Rubella (strain F-Therien) virus-like particles consisting of spike glycoprotein E1, spike glycoprotein E2 and Capsid protein(Uniprot accession number NP_062884.1), produced in mammalian HEK293 cells. VLPs were purified from culture supernatant by isopycnic centrifugation followed by dialysis.
Tag/Conjugate
Unconjugated
Alternative Names
RuV VLP
Format
Liquid, 0.2um filter sterilised.
Buffer
40mM Tris-HCl, pH 8.0, 100mM NaCl
Storage
Short term: 2-8°C; Long term: -80°C. Can be frozen, avoid multiple freeze/thaw cycles. Visible precipitates generated by freeze-thawing can be removed by centrifugation. This won't affect target protein content.
Stability
At 4°C: Not determined. At -80°C: Not determined.
Introduction
Rubella virus is an enveloped, positive single-stranded RNA virus and a member of the genus Rubivirus, which belongs to the Togaviridae family. It consists of three structural proteins: a capsid protein and two membrane-spanning glycoproteins, E1 and E2, localized in the virus envelope. E1 and E2 exist as a heterodimer and form the viral spike complexes on the virion surface. Formation of an E1-E2 heterodimer is required for transport of E1 out of the endoplasmic reticulum lumen to the Golgi apparatus and plasma membrane. E1 is the dominant surface molecule of the virus particle representing the main target for the detection and subsequent elimination of virus by the host's immune system. Although both E1 and E2 provide lifelong immunity, hemagglutination (HA) and viral neutralization (VN) is believed to be mainly targeted to E1 protein epitopes.
Keywords
Rubella Virus virus-like particles; Rubella Virus; RuV VLP; RuV; VLP
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