Rubella IgG ELISA Kit (DEIA011)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma
Species Reactivity
Human
Intended Use
Enzyme immunoassay based on microtiter plate for the detection and quantitative determination of human IgG antibodies against Rubella in serum and plasma
Storage
Store the components of the kit at 2-8C. After usage put the plate in the plastic bag, close the bottles with their screw caps, and again store the kit at 2-8C. After the first opening the kit should be used within 3 months, the diluted wash buffer can be kept for 4 weeks at 2-8C.
Precision
Intra-Assay-Precision: 4.3 – 7.2 %
Inter-Assay-Precision: 2.6 – 17.0 %
Inter-Lot-Precision: 5.3 – 23.2 %
Detection Range
10 – 500 IU/mL
Sensitivity
0.29 IU/mL
General Description
Rubella infection belongs to the classical children ́s diseases with a life-long immunity, and the virus is spread worldwide endemically. In non-vaccinated populations, 80-90% of the infections occur during the childhood. In spite of the rubella vaccination, introduced in 1974, in Germany there continue to appear connatal diseases.
The causative agent is a genetically stable RNA virus, which belongs to the genus rubivirus within the family of togaviridae. Human beings are the only known natural hosts for the rubella virus. The transmission occurs via droplet infection, with an incubation time of 14-23 days.
Clinically the disease manifests itself like a light flu infection. The nucal and retroaurical lymph nodes are swollen, and a moderate enlargement of the spleen is observed. A short and medium raise of temperature appears together with a rather slight sensation of illness. Rubella is overcome easily with insignificant and light symptoms during the childhood, however more attention is required in the case of the infection of non-immunized pregnant women, because of the possible malformations of the foetus, which can be generated. As the infection can be transmitted via the placenta, the developing foetus can suffer severe damages, the frequency and gravity of which is dependent on the moment of infection during pregnancy. A rubella infection during the 1st till 4th month can lead to a spontaneous abortion or premature birth. Since a specific causal therapy does not exist, the secondary signs like fever, arthritis or arthralgies are treated symptomatically.
The clinical differential diagnosis is problematic, because similar exanthems and feverish illnesses appear also in the course of other children ́s diseases like measles, scarlet and parvovirusitis.
The following laboratory methods are available: hemagglutination inhibition test (HIT), hemolysis- in-gel test or ELISA. The detection of virus-specific IgM antibodies is important for the assessment of fresh infections, and the IgG test is used for the determination of immunity. In the case of severe connatal infections, the isolation of the rubella virus from pharyngeal lavage, urine and other secretions can also be performed.

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