Recombinant Japanese Encephalitis Virus virus-like particles antigen

Product Overview

Recombinant JEV virus-like particles (strain SA-14) composed of pre-Membrane and Envelope proteins (NCBI accession number P27395.1, AA105-819) expressed in HEK293 cells and purified from culture supernatant using density gradient centrifugation and ion exchange chromatography.

Target

JEV VLP

Nature

Recombinant

Tag/Conjugate

Unconjugated

Alternative Names

JEV VLP

Format

Liquid

Concentration

0.88mg/ml

Buffer

Dulbecco's PBS pH7.4, sterile filtered

Preservative

None

Storage

Short term: 2-8°C; Long term: -80°C. Can be frozen, avoid multiple freeze/thaw cycles.

Stability

Not tested

Ship

Dry ice

Introduction

The Japanese encephalitis virus (JEV) is a flavivirus and is related to the West Nile Virus. JEV is the main cause of viral encephalitis in many countries of Asia with an estimated 68 000 clinical cases every year (WHO). Although symptomatic Japanese encephalitis (JE) is rare, the case-fatality rate among those with encephalitis can be as high as 30%. Permanent neurologic or psychiatric sequelae can occur in 30%–50% of those with encephalitis. Flaviviruses are positive-stranded RNA viruses with a 9-12- kb genome that is translated as a single polyprotein and then cleaved by host and viral proteases into structural capsid (C), premembrane (prM), envelope (E) proteins and seven nonstructural proteins. Capsid binds to viral RNA to form a nucleocapsid that is enveloped by an endoplasmic reticulum-derived membrane containing E and prM. E proteins are responsible for cellular attachment and possess a hydrophobic loop that mediates fusion of viral and host membrane.The E protein is therefore the primary target of neutralizing antibodies.

Keywords

Japanese Encephalitis Virus virus-like particles; JEV VLP; JEV; VLP