Intended Use
The JE IgG test for exposure to Japanese Encephalitis Virus (JEV) is an ELISA assay system for the detection of antibodies in human serum to JEV derived recombinant antigen (JERA).
It is not intended to screen blood or blood components and is for research use only. This kit has not been optimized for vaccine induced seroconversion studies.
Contents of Kit
Warning: Do not use any reagents where damage to the packaging has occurred.
The JE IgG ELISA kit contains sufficient reagents for one plate of 96 wells (12 x 8 strips) each. The kit contains the following reagents:
1. Coated Microtiter Strips for JE Human IgG: Strip holder in ziplock foil, containing 96 polystyrene microtiter wells coated with monoclonal antibody bound to recombinant JERA (Rows A, B, C, and D) and Control antigen NCA (Rows E, F, G and H). Store at 2-8°C until ready to use.
2. Sample Dilution Buffer for IgG: Two bottles, 25 mL, for serum sample dilution. Store at 2-8°C until ready to use.
3.JE IgG Positive Control: One vial, 50 μL. The JE IgG Positive Control will aid in monitoring the integrity of the kit. Store at 2-8°C until ready to use.
4. JE Negative Control: One vial, 50 μL. The JE Negative Control will aid in monitoring the integrity of the kit. Store at 2-8°C until ready to use.
5. 100× Conjugate for JE IgG: One vial, 150 μL, containing horseradish peroxidase (HRP) labeled goat anti-human IgG conjugate. Store at 2-8°C until ready to use.
6. Conjugate Dilution Buffer Type B: One bottle, 12 mL, containing a conjugate dilution buffer to dilute the 100× conjugate before adding to the ELISA plate. Store at 2-8°C until ready to use.
7. 10× Wash Buffer: One bottle, 120 mL of Wash Buffer to be used in all the washing steps of this procedure. Store at 2--8°C until ready to use.
8. EnWash: One bottle, 20 mL of EnWash to be used in between the washing steps after the addition of enzyme conjugate-HRP of this procedure. Store at 2-8C until ready to use.
9. Liquid TMB Substrate: One bottle, 9 mL of liquid substrate to be used in this procedure. Store at 2-8°C until ready to use. Note: The substrate should be kept in a light-protected bottle at all times as provided.
10. Stop Solution: One bottle, 6 mL to be used to stop the reaction. Store at 2-8°C until ready to use. Caution: Stop Solution contains strong acid. Wear protective gloves, mask and safety glasses. Dispose of all materials according to safety rules and regulations.
COA
Publication 
Loading ......
