Recombinant Human Glomerular Basement Membrane [His]

Nature

Recombinant

Tag/Conjugate

His

Molecular Weight

44 kDa

Purity

> 85% as determined by SDS-PAGE

Format

Liquid

Buffer

50mM Tris-HCl, 200mM NaCl, 4M Urea

Preservative

None

Storage

Store at -20°C to -80°C

Introduction

Antibodies to GBM can cause glomerulonephritis or pulmonary hemorrhage; the combination of these 2 clinical symptoms is known as Goodpasture's syndrome. The best known autoantigen in anti-GBM nephritis is the so-called Goodpasture antigen.
In 1984, the antigen was biochemically characterized and shown to be a 29 kDa collagenase-resistant molecule of the glomerular basement membrane. The important epitope is at the C-terminal end of type IV collagen and was later shown to be localized to the NC1 domain of the α3(IV) chain.

Keywords

Glomerular Basement Membrane; GBM; Type IV collagen; ANCA