Recombinant HAV VP1 Protein [GST]

Name: Recombinant HAV VP1 Protein [GST]
SKU: DAG542
Rating: 5 (1 reviews)

HAV VP1 [GST], recombinant protein from E. coli

COA

SDS

Datasheet

Summary

Product Overview

Recombinant Hepatitis A Virus VP1 antigen, contains the the VP1 immunodominant region and a GST fusion partner, has the molecular weight of 39kDa, was expressed in E. coli, and puried in vitro using conventional chromatography techniques.

Nature

Recombinant

Tag/Conjugate

GST

Molecular Weight

39 kDa

Purity

> 90% pure (10% PAGE coomassie staining). Inclusion Bodies

Format

Purified, Liquid

Concentration

1 mg/ml

Buffer

10mM Carbonate-bicarbonate buffer, pH 9.6, 0.1% SDS containing 50% glycerol

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

Hepatitis A virus (HAV) is the sole member of the Hepatovirus genus within the family Picornaviridae. The capsid of HAV encloses a single-stranded RNA genome of about 7.5 kb which is translated into a single polyprotein. The virion proteins VP1 to VP4 and the nonstructural proteins are generated from the polyprotein by a cascade of proteolytic cleavages. Only one protease, viral protease 3C, has been implicated in the nine protein scissions. Processing of the capsid protein precursor region generates a unique intermediate, PX (VP1-2A), which accumulates in infected cells and is assumed to serve as precursor to VP1 found in virions, although the details of this reaction have not been determined. Capsid proteins VP1, VP2, and VP3 form a closed capsid enclosing the viral positive strand RNA genome. VP1 is a major viral antigen.

Antigen Description

Hepatitis A virus (HAV) encodes a single polyprotein which is posttranslationally processed into the functional structural and nonstructural proteins. Only one protease, viral protease 3C, has been implicated in the nine protein scissions.The protein contains the HAV VP1 immunodominant regions, amino acids: (502-605). Hepatitis A Virus 1 protein is purified by proprietary chromatographic techniques.

Keywords

HAV; HAV VP1-P2A; Hepatitis A Virus VP1-P2A; Picornaviridae; Hepatovirus

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References

Molecular epidemiology of hepatitis A in Noord-Brabant, the Netherlands

JOURNAL OF CLINICAL VIROLOGY

Authors: Tjon, GMS; Wijkmans, CJ; Coutinho, RA; Koek, AG; van den Hoek, JAR; Leenders, ACAP; Schneeberger, PM; Bruisten, SM

Abstract

Background: Previous studies on the molecular epidemiology of hepatitis A virus (HAV) in Amsterdam, The Netherlands, show that subgenotype 1A is mainly seen among homosexual men practising anonymous oral-anal sex in saunas and darkrooms, while subgenotype I B is usually detected among children originating from Morocco, and subgenotype 3A is mostly found among travellers to Pakistan. Objective: We studied the genotype distribution in a more rural area of The Netherlands, Noord-Brabant, and compared it with Amsterdam. Study design: We collected blood and feces samples from 34 HAV IgM(+) individuals who were reported from August 2001-March 2003 at the Municipal Health Service (MHS) Heart for Brabant (Brabant). We also collected feces samples from nine household contacts of whom the HAV IgM status was not known. HAV RNA was isolated and subsequently amplified by reverse transcriptase polymerase chain reaction (RT-PCR) at the VP1-P2a and the VP3-VP1 region, sequenced and analysed. Results and conclusions: In most cases, relations between risk groups and HAV subgenotypes in Noord-Brabant were similar to those in Amsterdam. Next to genotypes I and 3 we also detected a genotype 2/7 strain in a Noord-Brabant case. Also, in contrast to the Amsterdam study, sporadic transmission occurred among various risk groups. Children involved in a school-related outbreak were infected with strains identical to one that was previously isolated from a man who has sex with men (MSM). Also, Dutch patients having no epidemiological link with Turkish or Moroccan children harboured strains imported from high-endemic countries. Furthermore, we report a special case in which HAV may be causally involved in meningitis. The results of this study show that the molecular epidemiology of HAV in The Netherlands can be more complicated than previously anticipated and that HAV phylogenetic studies can provide important information for the design of appropriate public health measures. (C) 2004 Elsevier B.V. All rights reserved.

Molecular characterisation of the hepatitis A virus circulating in the 2001-2005 outbreaks in Thailand

ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY

Authors: Barameechai, K.; Sa-Nguanmoo, P.; Suwannakarn, K.; Thongmee, C.; Payungporn, S.; Chongsrisawat, V.; Theamboonlers, A.; Poovorawan, Y.

Abstract

In a recent study of hepatitis A virus (HAV) in Thailand, viral isolates recovered during several outbreaks of infection that occurred between 2001 and 2005 were genotyped and subjected to phylogenetic analysis. Anti-HAV IgM was detected, by ELISA, in many of the 283 serum samples that were collected from the provinces of Suphanburi, Songkhla, Chiangrai and Lampang: 40 (48.2% of those investigated), 38 (47.5%), 25 (41.0%) and 32 (54.2%), respectively. The HAV RNA in the positive samples was reverse transcribed and amplified, using a nested PCR focussed on the VP1-2A region, before the nucleotides of the VP1-2A region of each HAV-RNA-positive sample were sequenced. All the isolates investigated clustered in subgenotype IA and, hence, are closely related to the strains previously investigated in Thailand. When the genome of one sample from an outbreak in Lampang (LP014) was fully sequenced, the results of genome comparison and phylogenetic analysis again indicated subgenotype 1A, which appears to be the predominant form of HAV circulating throughout Thailand.