Recombinant HAV VP1 (a.a. 502-605)

Name: Recombinant HAV VP1 (a.a. 502-605)
SKU: DAG3317
Rating: 5 (1 reviews)

HAV VP1 (aa 502-605), recombinant protein from E. coli

COA

SDS

Datasheet

Summary

Product Overview

Recombinant HAV VP1

Specificity

The E. coli derived 48 kDa recombinant protein contains the VP1 immunodominant region (amino acids 502-605). It is immunoreactive with sera HAV-infected individuals. The HAV VP1 protein was purified by proprietary chromatographic techniques. It is >90% pu

Nature

Recombinant

Tag/Conjugate

Unconjugated

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

HepatitisA Virus (HAV) is a 27nm nonenveloped, spherical, positive stranded RNA virus, classified within the genus hepatovirus of the picornavirus family and isamong the smallest and structurally simplest of the RNA animal viruses. Asingle large polyprotein is expressed from a large open reading frameextending through most of the genomic RNA. This polyprotein is subsequentlycleaved by a viral protease (3Cpro) to form three (possibly four) capsidproteins and several nonstructural proteins. HAV genomic replication occursexclusively in the cytoplasm of the infected hepatocyte by a mechanisminvolving an RNA-dependent RNA polymerase.

Keywords

HAV; Hepatitis A Virus; Picornaviridae; Hepatovirus; Hepatitis A

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Documents

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References

IDENTIFICATION AND CHARACTERIZATION OF INCOMPLETE HEPATITIS-A VIRUS-PARTICLES

JOURNAL OF GENERAL VIROLOGY

Authors: RUCHTI, F; SIEGL, G; WEITZ, M

Abstract

The range of hepatitis A virus (HAV) particles generated during persistent infection of different cell lines was studied. Buoyant density and sedimentation analyses of cell extracts revealed a uniform profile of particles in all cell lines analysed except for BS-C-1' cells. The virion itself usually represented less than 50% of the total mass of virus antigen. A major portion of the antigen was associated with non-infectious, empty particles, which banded at 1.305 g/ml and 1.20 g/ml CsCl, and sedimented in sucrose gradients at 76S and 59S. Empty HAV particles were similar to those of poliovirus with respect to their physical stability and had the characteristic capsid protein content (VP0, VP1 and VP3). An additional RNA-containing particle, probably the provirion, represented only a minor species characterized by a buoyant density of 1.32 g/ml in CsCl and sedimenting at 130S.

Cocirculation of and coinfections with hepatitis A virus subgenotypes IIIA and IB in patients from Pune, western India

HEPATOLOGY RESEARCH

Authors: Chitambar, Shobha; Joshi, Madhuri; Lole, Kavita; Walimbe, Atul; Vaidya, Sunil

Abstract

Aim: During the 1990s, a changing pattern of epidemiology of hepatitis A was reported in different populations of India. The present study was undertaken to investigate the molecular epidemiology of hepatitis A virus (HAV) strains over a period of 10 years. Methods: Stool/serum samples were collected from hepatitis A patients clinically presenting acute viral hepatitis and hepatic encephalopathy. Reverse transcriptase polymerase chain reaction (RT-PCR) was performed to detect HAV-RNA. HAV genomes were examined by sequencing PCR products of VP1/2A junction (168 bp) and RNA polymerase (116 bp) regions. Results: Subgenotype IIIA and IB were detected in 74.2% and 9.7% of specimens, respectively, while 16.1% of patients had mixed infections. Sewage samples also showed presence of both IIIA (9/10) and IB (1/10) subgenotypes. RNA polymerase region showed two clusters constituting 51.6% and 19.4% strains closer to Nor21 and HM175 strains, respectively, in clinical specimens. Three isolates appeared as discordant subgenotypes in VP1/2A and RNA polymerase regions. Conclusions: The data revealed cocirculation of and coinfection with subgenotypes IIIA and IB, with predominance of IIIA and genetic heterogeneity of HAV strains in western India.