Recombinant E. coli DnaJ Protein (a.a. 1-376)

Product Overview

Escherichia coli dnaJ (NP_414556, 1 a.a. - 376 a.a.) full-length recombinant protein expressed inEscherichia coli .

Nature

Recombinant

Tag/Conjugate

Unconjugated

Purity

Conventional Chromatography

Format

Liquid

Concentration

1 mg/ml

Buffer

In 25 mM Tris-HCl, 100 mM NaCl, pH 7.5 (5 mM DTT, 10% glycerol)

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

DnaK and DnaJ both bind to the same target peptide, forming a ternary complex. The DnaK system of Escherichia coli is a homolog of the eukaryotic Hsp70 chaperone system.

Antigen Description

The E. coli heat-shock protein DnaJ has been implicated in protein folding and protein complex dissociation. DnaJ, Heat shock protein, functions in association with DnaK(Hsp70) molecular chaperone to facilitate protein folding. p70 chaperone. DnaJ plays a key role in the chaperone reaction by stimulating the ATPase activity and activating the substrate binding of Hsp70. DnaJ consists of four domains that are N-terminal 76 amino acid J-domain, G/F domain, zinc-binding cystein rich CR-domain, C-terminal CTD-domain and they are conserved to various degrees among the homologues.