E. coli O157 ELISA Kit

Name: E. coli O157 ELISA Kit
SKU: DEIA2437
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Food, Fecal or Environmental Samples

Detection Method

sELISA

Intended Use

The E. coli O157 ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the E. coli O157. The ELISA antibodies specifically recognize the E.coli O157 strain, will not react with other E. coli strains with different O antigens and other types of bacteria such as Shigella sonnei, Pseudomonas aeruginosa and Salmonella flexneri. The ELISA kit has a detection sensitivity limit of 1.2 × 10⁴ CFU equivalents/mL E. coli O157. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and food test samples.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Anti- E.coli O157 Antibody Coated Plate	One strip well 96-well plate	4°C
2	FITC-Conjugated Anti- E.coli O157 Monoclonal Antibody	One 20 μL vial	4°C
3	HRP-Conjugated Anti-FITC Monoclonal Antibody	One 20 μL vial	4°C
4	Assay Diluent	One 50 mL bottle	4°C
5	10X Wash Buffer	One 100 mL bottle	4°C
6	Substrate Solution	One 12 mL amber bottle	4°C
7	Stop Solution	One 12 mL bottle	4°C
8	Heat Killed E.coli O157 Standard	One 200 μL vial	-20°C

Storage

Upon receiving, aliquot and store heat killed E.coli O157 Standard at -80°C and avoid freeze/thaw. Store all other components at 4°C.

General Description

E. coli is one of the many groups of bacteria that live in the intestines of healthy humans and most warm-blooded animals. E. coli's serotyping is based on the flagellar (H) and polysaccharide (O) antigens.
Shiga toxin–producing E.coli strains are major foodborne pathogens and have caused a large number of human illnesses. E.coli O157:H7 is the most well-known serotype and it can cause diarrhea, hemorrhagic colitis, and in more severe cases hemolytic uremic syndrome. Most E.coli O157:H7 infection outbreaks have been linked to the consumption of ground beef.

Standard Curve

The following figures demonstrate typical E.coli O157 ELISA results. One should use the data below for reference only. This data should not be used to interpret actual results.

Figure 1: Heat Killed E.coli O157 ELISA Standard Curve.

Figure 2: E.coli O157 ELISA Selectivity. Heat killed 5 × 10⁸ equivalent CFUs/mL bacterial culture was diluted in Assay Diluent and subjected to E.coli O157 ELISA Kit according to Assay Protocol.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Q & A

Ask a Question

Q: Is this product suitable for testing water samples?

A: We have never evaluated DEIA2437 with water samples before.
Water can affect antigen-antibody interactions in ELISAs in general.
It may be necessary to dilute the water with assay diluent to avoid using pure water.
I recommend that you perform a sample buffer compatibility test. You can make a standard curve in two ways: (a) in assay diluent; (b) in sample buffer.
Then run these two standard curves side by side to evaluate the signal.
It is recommended to perform spike recovery on the samples. You can spike the samples with a known amount of heat-killed E. coli O157 standard (contact Creative Diagnostics for standards) and check the recovery.
It is also recommended to run the samples without spiking. As a control, you may consider spiking an equal amount of the standard in the assay diluent.

Customer Reviews