Rat Cpt1a (Carnitine O-palmitoyltransferase 1, liver isoform) ELISA Kit (DEIA-FN319)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma, cell culture supernatants, tissue homogenate
Species Reactivity
Rat
Intended Use
For quantitative detection of Rat Cpt1a (Carnitine O-palmitoyltransferase 1, liver isoform) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
15.6-1000 pg/mL
Sensitivity
9.375 pg/mL
Standard Curve

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References


Cheng, Y; Dharancy, S; et al. Hepatitis C virus infection down-regulates the expression of peroxisome proliferator-activated receptor alpha and carnitine palmitoyl acyl-CoA transferase 1A. WORLD JOURNAL OF GASTROENTEROLOGY 11:7591-7596(2005).
Roomets, E; Kivela, T; et al. Carnitine palmitoyltransferase I and Acyl-CoA dehydrogenase 9 in retina: Insights of retinopathy in mitochondrial trifunctional protein defects. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 49:1660-1664(2008).

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