Pyruvate ELISA Kit

Sample

Serum, plasma, blood, urine, and saliva

Species Reactivity

Universal

Detection Method

ELISA

Intended Use

Enzyme immunoassay for determination of Pyruvate.

Contents of Kit

Kit will arrive packaged as a -20°C kit. For best results, store the kit as supplied or remove components and store as stated below.
1. Pyruvate Assay Buffer (10X): 1 vial/10 ml, -20°C
2. Pyruvate Assay Cofactor Mixture: 2 vials, -20°C
3. Pyruvate Assay Enzyme Mixture : 2 vials, -20°C
4. Pyruvate Assay Detector: 3 vials, -20°C
5. Pyruvate Assay Standard: 2 vials, -20°C
6. DMSO Assay Reagent: 1 vial/1 ml, RT
7. MPA Assay Reagent: 1 vial/2 g, RT
8. Potassium Carbonate Assay Reagent: 1 vial/5 ml, -20°C
9. 96-Well Plate (black): 1 plate, RT
10. 96-Well Cover Sheet: 1 cover, RT
If any of the items listed above are damaged or missing, please contact our Customer Service department.

Storage

This kit will perform as specified if stored at -20°C and used before the expiration date indicated on the outside of the box.

Precision

When a series of eight blood and eight plasma measurements were performed on the same day, the intra-assay coefficients of variation were 1.2 and 2.4%, respectively. When a series of eight blood or eight plasma measurements were performed on six different days under the same experimental conditions, the inter-assay coefficients of variation were 2.0 and 2.8%, respectively.

Sensitivity

The Lower Limit of Quantification (LLOQ) for the fluorometric assay is 1.5 μM.
The LLOQ for the colorimetric assay is 3.0 μM.
The Lower Limit of Detection (LLOD) for both assays is 0.75 μM.

General Description

Pyruvate is a key intermediate in cellular metabolism. It is formed primarily from glucose via glycolysis but can also be derived from certain amino acids and lactate. Pyruvate is converted by the pyruvate dehydrogenase complex into acetyl CoA, which enters the citric acid cycle to produce ATP. The NADH formed by this conversion is used for mitochondrial electron transport and β-oxidation. Pyruvate can also be converted back into glucose via gluconeogenesis when sufficient oxygen is present. When oxygen is not present, pyruvate is converted into lactate.
Pyruvate accumulates in the blood and urine of individuals with pyruvate dehydrogenase deficiency, an inborn error of metabolism characterized by mutations in the genes encoding pyruvate dehydrogenase complex enzymes, that leads to mild-to-severe metabolic or neurological impairments. Defects in pyruvate metabolism also play a role in several disease states, including cancer, neurodegenerative diseases, and heart failure.
The lactate-to-pyruvate ratio reflects the redox state of the cell and describes the balance between NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase. Defects in pyruvate metabolism can be identified by an increase in blood lactate in conjunction with a normal lactate-to-pyruvate ratio, and defects in mitochondrial electron transport are evidenced by an increased lactate-to-pyruvate threshold.