Recombinant Vesicular Stomatitis Virus pseudotyped Ebola glycoprotein (rVSV pseudotyped EBOV GP) system in which the G protein of VSV has been deleted, replaced with firefly luciferase and used to produce VSV pseudotypes containing the envelope glycoprotein of Ebola virus. Since the infectivity of rVSV pseudotyped EBOV GP is restricted to a single round of replication, analyses of viral entry can be performed using just biosafety level 2 (BSL-2) containment. Infectivity and neutralization of infectivity can be measured by luciferase activity.
DMEM, 1% FBS, L-glutamine and Penicillin/Streptomycin
Storage
Store at -80⁰C . Multiple freeze/thaw cycles not recommended. When using the virus, transfer the virus from the -80⁰C refrigerator and melt it in an ice bath.
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