Automatic and sensitive detection of West Nile virus non-structural
protein 1 with a portable SERS–LFIA detector
Applications: LFIA
Reactive species: Ebola Virus
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Abstract: A portable surface-enhanced Raman scattering (SERS)-lateral flow immunoassay (LFIA) detector has been developed for the automatic and highly sensitive detection of West Nile virus (WNV) non-structural protein 1 (NS1) and actual WNV samples. Au@Ag nanoparticles (Au@Ag NPs) labeled with double-layer Raman molecules were used as SERS tags to prepare WNV-specific SERS-LFIA strips. On this platform, the WNV-specific antigen NS1 protein was quantitatively and sensitively detected. The detection limit for the WNV NS1 protein was 0.1 ng/mL, which was 100-fold more sensitive than visual signals. The detection limit for inactivated WNV virions was 0.2 × 102 copies/μL. The sensitivity of the SERS-LFIA detector was comparable to that of the fluorescence quantitative reverse transcription-polymerase chain reaction assay. The prepared SERS-LFIA strips exhibited high sensitivity and good specificity for WNV. Thus, the strips developed herein have clinical application value. Moreover, the portable SERS-LFIA detector enabled automatic and rapid detection of the SERS-LFIA strips. The platform established herein is expected to make a substantial contribution to the diagnosis and control of outbreaks of emerging infectious diseases, including WNV."
Article snippet:
Ebola nucleoprotein antigen (
Catalog No. DAGA-3013) was purchased from Creative Diagnostics (Shirley, NY, USA, https://www.creative-diagnostics.com)
Figure 1. Results of specificity test of the WNV-specific SERS–LFIA strips. A: Histogram of Raman signal intensities on the T lines. B: Raman spectra on the T lines.
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