Protein A ELISA Kit

Sample

Various biological products

Detection Method

sELISA

Intended Use

The Protein A ELISA kit (enzyme-linked immunosorbent assay) is a highly sensitive kit for detecting Protein A residues. It can accurately identify multiple imported and domestically produced Protein A variants. This kit enables sensitive, specific, and accurate detection of Protein A residues in intermediate, semi-finished, and finished products of various biological products. It also facilitates Protein A affinity resin manufacturers to monitor the shedding of Protein A under specific conditions.

Contents of Kit

1. Microplate: 96 well polystyrene microplate (12 strips of 8 wells).
2. Standard 1 MabSelect SuRe (Cytiva): 1×300 μL, 50 ng/mL.
3. Standard 2 MabSelect PrismA (Cytiva): 1×300 μL, 50 ng/mL.
4. Standard 3 Recombinant Protein A: 1×300 μL, 50 ng/mL.
5. Standard 4 MaXtar® ARPA Ligand Protein A: 1×300 μL, 50 ng/mL.

6. Detection Antibody: 1×15 mL, antibody conjugated to horseradish peroxidase (HRP) with preservatives.
7. Sample Dilution 20×: 30 mL of a 20-fold concentrated solution.
8. Wash Buffer 20×: 30 mL of a 20-fold concentrated solution.
9. Chromogen Solution A: 8 mL, avoid direct light.
10. Chromogen Solution B: 8 mL, avoid direct light.
11. 3 Cover foil.

Storage

1. Store all reagents at 2-8°C, and not be frozen or thawed. The product is valid for 12 months.
2. All reagents must be brought to room temperature (20-25°C) prior to use.

Performance Characteristics

Intra-assay Precision (Precision within an assay): CV% < 10%
Inter-assay Precision (Precision between assays): CV% < 15%
Spike Recovery: 80% - 120%

Detection Range

5.12 - 500 pg/mL

Detection Limit

＜ 5.12 pg/mL

Sensitivity

5.12 pg/mL

General Description

Product Features:

1. High versatility, suitable for detecting various recombinant and alkali-resistant Protein A variants.
2. The coating antibody and the detection antibody are polyclonal antibodies from chickens, reducing the spatial binding of antibody samples with Protein A.
3. Steric hindrance effects are minimized to ensure scientific validity in principle.
4. The sample pretreatment involves acid treatment, and after sample dilution, direct sample addition reaction without cumbersome steps such as heating or centrifugation, making the operation simple.
5. High sensitivity in detection.

Standard Curve

1. MabSelect SuRe (Cytiva)

2. MabSelect PrismA (Cytiva)

3. Recombinant Protein A

4. MaXtar® ARPA Ligand Protein A

General Notes

Other ELISA Kits