Alkali-Resistant Protein A ELISA Kit

Name: Alkali-Resistant Protein A ELISA Kit
SKU: DEIA-BY019
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Biopharmaceutical samples

Detection Method

Sandwich-ELISA

Intended Use

This product is a universal detection kit used for quantitative detection of residue Alkali Resistant Protein A (MabSelect SuReTM Protein A) concentration in biopharmaceutical samples.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Protein A Microwell Plate	12×8	2-8°C
2	Alkali-Resistant Protein A Standard	3 vials, lyophilized	2-8°C
3	Protein A 100× HRP-Antibody	2×30 μL	2-8°C.
4	Assay Diluent	2×25 mL	2-8°C.
5	20× Wash Buffer Concentrate	30 mL	2-8°C.
6	Substrate Solution	12 mL	2-8°C in the dark.
7	Stop Solution	12 mL	2-8°C.
8	Adhesive plate cover	2 sheets

Storage

Stored at 2-8°C until the expiration date.

Precision

Intra-assay Precision (Precision within an assay): ≤10%. Inter-assay Precision (Precision between assays) : ≤10%.

Sensitivity

LOD: 6.6 pg/mL
LOQ: 13.7 pg/mL

General Description

Protein A affinity chromatography resin is a commonly used tool for separating and purifying antibodies in the production and purification process of antibody products. However, during the antibody purification process, Protein A may leach, resulting in the presence of Protein A in the antibody products, which affects the purity and potency of the products and brings significant problems to the pharmaceutical industry.
Protein A includes natural Protein A, recombinant Protein A, and alkali resistant recombinant Protein A with significantly different structures from natural Protein A, such as GE's MabSelect SuReTM Protein A.

Standard Curve

This standard curve is for demonstration only. A standard curve must be generated for each assay.