ZIKV IgM Capture ELISA Kit

Sample

serum

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The ZIKV IgM Capture ELISA is intended for the qualitative detection of Zika virus IgM antibodies in human sera.

Contents of Kit

Warning: Do not use any reagents when damage to the packaging has occurred.
The ZIKV IgM Capture ELISA contains sufficient reagents for one plate of 96 wells (12 × 8 strips) for human IgM targeting Zika virus. This is sufficient for testing a maximum of 28 unknown samples for human IgM with controls included in duplicate.
Below is a list of the kit contents.
1. Coated Microtiter Test Strips for IgM (1 plate containing twelve 1×8 strips for human IgM): ELISA plate strip holder with 96 (12×8 strips) polystyrene microtiter wells pre-coated with capture antibodies specific for human IgM. Store at 2-8°C until expiry
2. ZIKV IgM Negative Control (1×50μL): The negative control aids in verifying the validity of the kit. Store at 2- 8°C until expiry. Centrifuge briefly prior to use to sediment any precipitate
3. ZIKV IgM Positive Control (1×50μL): The positive control aids in verifying the validity of the kit. Store at 2-8°C until expiry. Centrifuge briefly prior to use to sediment any precipitate
4. ZIKV Sample Dilution Buffer (1×25mL): This buffer solution is used for diluting all serum samples and controls prior to testing in the ELISA. Store at 2-8°C until expiry.
5. Ready-To-Use ZlKV Recombinant Antigen for IgM (1×3mL): This vial contains ready-to-use (RTU) ZIKV antigen (Zika Ag) that comprises the Zika envelope glycoproteins. Store at 2-8°C until expiry
6. Cross-reactive Control Antigen for ZlKV IgM (1×3mL): This vial contains a cross-reactive control antiger (CCA) cocktail. This is used to aid in the interpretation of the ELISA results. Store at 2-8°C until expiry
7. Normal Cell Antigen for ZIKV IgM (1×3mL): This vial contains a normal control antigen (NCA). This is used to aid in the interpretation of the ELISA results. Store at 2-8°C until expiry
8. Ready-To-Use Secondary Antibody (1×9mL): This vial contains secondary antibodies targeting the flavivirus antigens. Store at 2-8°C until expiry
9. 100× Conjugate for ZIKV IgM (1×150μL): This vial contains horseradish peroxidase-labeled anti-mouse antibody. Mix well prior to use. The 100X Conjugate is added to the Conjugate Diluent before use. Store the undiluted 100X conjugate at 2-8°C until expiry
10. Conjugate Diluent for ZIKV (1×9mL): This solution is used to dilute the 100X conjugate before adding to the ELISA plate. Store at 2-8°C until expiry
11. 10× Wash Buffer (1×120mL): One bottle of 10× concentrated Wash Buffer is used as directed in Test Procedure. Store at 2-8°C until expiry
12. Liguid TMB Substrate (1×12mL): Chromogenic substrate that reacts to horseradish peroxidase to generate the optical signal measured by the ELISA spectrophotometer. The substrate is light sensitive. Store at 2-8°C until expiry.
13. Stop Solution (1×9mL): Is used to terminate the reaction as directed in the Test Procedure. Store at 2-8°C until expiry. Caution: strong acid, wear protective gloves, mask and safety glasses. Dispose of all the materials according to safety rules and regulations.

Storage

2-8°C