Zika Virus E Protein Human IgM ELISA Kit

Sample

Semi-quantitative

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The Zika Virus E Protein Human IgM ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgM antibody against pathogen antigen(s) in human serum or plasma. This ELISA kit is for research use only, not for therapeutic or diagnostic applications.

Contents of Kit

1. ZIKVE coated 96-well Microplate 96 wells, (12 strips x 8 wells) coated with ZIKVE. Storage / Stability After Preparation: 1 month at 4°C*
2. Wash Buffer Concentrate, 25 ml of 20× concentrated solution. Storage / Stability After Preparation: 1 month at 4°C
3. Positive Control, 1 vial of Positive Control sample containing anti-pathogen antigen antibody. Storage / Stability After Preparation: 1 week at -80°C
4. Biotinylated Anti-Human IgM, 1 vial of solution. Storage / Stability After Preparation: 5 days at 4°C
5. HRP-Streptavidin concentrate, 1 vial of solution. Storage / Stability After Preparation: Do not store and reuse
6. TMB One-Step Substrate Reagent, 12 ml of 3,3',5,5'-tetramethylbenzidine (TMB buffer solution). Storage / Stability After Preparation: 1 month at 4°C
7. Stop Solution, 8 ml of 0.2 M sulfuric acid. Storage / Stability After Preparation: N/A
8. Assay Diluent B, 15 ml of 5× concentrated buffer. Storage / Stability After Preparation: 1 month at 4°C
9. 5× Sample Diluent, 25 ml of 5× diluent buffer, 0.5% proclin 300 as preservative. Storage / Stability After Preparation: 1 month at 4°C
Note 1. Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
Note 2. Components in final, validated assay may differ from those of preliminary manual. Please consult final manual prior to beginning experiment.

Storage

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see table below

Standard Curve