Total Collagen Assay Kit

Name: Total Collagen Assay Kit
SKU: DFLA26239
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Urine, Serum, Plasma, Tissue extracts, Other biological fluids

Species Reactivity

Species-independent

Detection Method

Enzymes Assay

Intended Use

Detection and quantification of total collagen content in urine, serum, plasma, tissue extracts, and other biological fluid samples.

Contents of Kit

Component	Quantity	Storage
96-Well Microplate	1
Substrate	Powder x 1	4°C, keep in dark
Substrate Diluent	8 mL x 1	4°C, keep in dark
Stop Solution	4 mL x 1	4°C
Dye Reagent	Powder x 1	4°C, keep in dark
Dye Reagent Diluent	4 mL x 1	4°C
Standard	Powder x 1	4°C
Plate Sealer	3
Instruction Manual	1

Storage

2 - 8°C

Detection Range

0.01 mg/mL - 1 mg/mL

General Description

Collagen is the key structural protein of connective tissue and the most abundant protein in mammals. It occurs in many different types and forms with Types I -V being the most common. Aside from the crucial role it plays in the body, it has numerous medical applications such as its use in reconstructive surgery including bone and skin grafts. It is also commonly used in cosmetics due to its anti-aging and skin healing properties. Total Collagen Microplate Assay Kit is a simple and sensitive assay to detect small amounts of collagens in a variety of samples. The assay is based on the acid hydrolysis of samples to form hydrolysates and Hydroxyproline. This released Hydroxyproline gets oxidized to form a reaction intermediate, which further in the reaction, forms a chromophore can be measured at a colorimetric readout at 560 nm.

Citations