FK-506 ELISA Kit

Sample

Plasma, Serum

Species Reactivity

Human

Detection Method

cELISA

Intended Use

FK-506 ELISA Kit is a competitive binding enzyme immunoassay for the quantitative measurement of FK-506.

Contents of Kit

1. 96-wells microtiter plate: Twelve strips of 8 detachable wells, coated with Anti-FK-506 antibody. (#S): 96 (12 × 8) wells
2. Calibrator: Containing 0, 2, 10 and 50 ng/mL of FK-506: 0.6 mL × 4
3. FK-506-Horseradish Peroxidase conjugate (ACC-HRP). (#3): 10.5 mL
4. Stabilized Tetramethylbenzidine (TMB) substrate. Ready to use. (#5): 10.5 mL
5. Wash Buffer (10× PBS-Tween). Dilute 10 fold with distilled or deionized water to 150 mL prior to use. (#6): 15 mL
6. Stop Solution, 3 N HCl. (#7): 10.5 mL

Storage

All reagents of the kit are stable, if stored at 2-8 °C, until the expiration date stated on the kit.

General Description

FK-506 (Tacrolimus) is a microlide lactone of fugal origin with potent immunosuppressive properties. FK-506 is used for primary or rescue immunosuppressant in patients after solid organ transplantation. Clinically relevant FK-506 side effects are nephrotoxicity, neurotoxicity, diabetes mellitus and hypertension. Most of these side effects are more closely related to FK-506 blood levels than dosage, therefore therapeutic drug monitoring of FK-506 levels is a prerequisite for therapy.

Standard Curve