Human SDMA ELISA Kit

Sample

Serum, Plasma-EDTA

Species Reactivity

Human

Detection Method

cELISA

Intended Use

This Creative Diagnostics assay is intended for the quantitative determination of SDMA in human serum and plasma-EDTA. For research use only. Not for use in diagnostic procedures.

Contents of Kit

1. MT-Strips
2. Standards 1-6
3. Control 1 & 2
4. Acylation Buffer
5. Acylation Reagent
6. Antiserum
7. Enzyme Conjugate
8. Wash Buffer
9. Substrate
10. Stop Solution
11. Equalizing Reagent
12. Solvent

Storage

Store the complete kit at 2-8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Performance Characteristics

For in vitro use only.
Disposable gloves should be used.
Material of animal origin used in the preparation of the kit has been obtained from animals certified as healthy but these materiále should be handled as potentially infectious.

Detection Range

0.2-3.0 μmol/l

Detection Limit

0.03 μmol/l

Sensitivity

0.03 μmol/l

General Description

Dosing of most drugs must be adapted in renal insufficiency, making accurate assessment of renal function an essential component of diagnostics in clinical medicine. Furthermore, even modest impairment of renal function has been recognized as a cardiovascular risk factor. As the most commonly used marker of renal excretory function, serum creatinine concentration, does not adequately respond to mild to moderate impairment of renal function, more sensitive markers for renal excretory function are urgently seeked, especially in mild stages of renal impairment. SDMA is a methylated derivative of the amino acid L-arginine (symmetric dimethylarginine). SDMA is eliminated from the body exclusively by renal excretion; therefore SDMA plasma concentration is tightly related to renal function.
Thus, quantification of plasma SDMA is an adequate means to assess renal function, as could be demonstrated in a series of recent clinical trials: In 18 clinical studies involving more than 2,100 patients systemic SDMA concentrations were highly correlated with inulin clearance as well as with various clearance estimates and better corresponded to mild renal function impairment than serum creatinine. Thus, SDMA exhibits properties of a reliable marker of renal function. Furthermore, there is evidence showing that elevated SDMA levels, as they may occur in renal function impairment, may prospectively indicate future risk of cardiovascular disease and mortality independently of the level of renal impairment.

Standard Curve